This guide is an introduction to using the new RNASeq functions in MeV. The guide contains a brief tour of the new RNASeq file loader and a demonstration of a few of the new functions we have added specifically to support RNASeq data. The guide will first walk you through loading the data using the new RNA-Seq file loader. Then it will describe using an RNA-Seq-optimized module, EdgeR, to find differentially expressed genes between two groups of samples. Finally, it will demonstrate how to examine these differentially expressed genes for functional themes using the new module GOSeq.

These new options were added in MeV v4.7. If you already have MeV v4.7 installed, you can skip the Setup step and go directly to Loading a Data Set.