Launch MeV by double-clicking on the TMEV.bat file (Windows), the tmev.sh file (Linux) or the MeV application icon (Mac OSX).

When you launch MeV, two windows will open. The small narrow window across the top of the screen is called the MeV main menubar. This window is used normally to open new MultipleArrayViewer windows and manage other MeV properties. We will not be using this menubar window for the purposes of this tutorial. The larger window that opens is called a MultipleArrayViewer (MAV). This is where the majority of our work will take place.

Download and unzip the file nease_example_files.zip. This file contains the expression data and supporting GO term files we will use to replicate the analysis presented in the manuscript.

Choose File-> Open Analysis from the MAV window. In the file chooser that opens, navigate to the folder where you unzipped nease_example_files.zip, and select the file ER_status_SAM_1_Miller.anl and choose Open. A saved analysis will be loaded into MeV. This may take some time.

These data are fully described in the manuscript. They are based on the data from Miller et al. (2005) and Minn et al. (2005).